Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Laser Microdissection-Based Protocol voor de…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Laser Microdissection-Based Protocol voor de LC-MS/MS analyse van het proteomische profiel van Neuromelanine Granules
Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
Journal (Neuroscience)
Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
Journal (Neuroscience)
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
 
Click here for the English version

Laser Microdissection-Based Protocol voor de LC-MS/MS analyse van het proteomische profiel van Neuromelanine Granules

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier wordt een robuust protocol gepresenteerd voor het isoleren van neuromelaninekorrels uit menselijk post-mortem substantia nigra pars compacta weefsel via lasermicrodissectie. Dit herziene en geoptimaliseerde protocol minimaliseert massaal de vereiste tijd voor monsterverzameling, vermindert de vereiste monsterhoeveelheid en verbetert de identificatie en kwantificering van eiwitten door LC-MS / MS-analyse.

Tags

Neurowetenschappen Uitgave 178 Neuromelaninekorrels lasermicrodissectie massaspectrometrie substantia nigra pars compacta parallelle reactiemonitoring data-afhankelijke acquisitie
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter