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JoVE Journal Neuroscience
Protocollo basato sulla microdissezione laser per l'analisi LC-MS/MS del profilo proteomico dei granuli di neuromelanina
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Protocollo basato sulla microdissezione laser per l'analisi LC-MS/MS del profilo proteomico dei granuli di neuromelanina

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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Un robusto protocollo è presentato qui per isolare i granuli di neuromelanina dal tessuto umano post-mortem substantia nigra pars compacta tramite microdissezione laser. Questo protocollo rivisto e ottimizzato riduce drasticamente il tempo necessario per la raccolta dei campioni, riduce la quantità di campione richiesta e migliora l'identificazione e la quantificazione delle proteine mediante analisi LC-MS/MS.

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Neuroscienze Numero 178 Granuli di neuromelanina microdissezione laser spettrometria di massa substantia nigra pars compacta monitoraggio delle reazioni parallele acquisizione dipendente dai dati
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