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Journal / Neuroscience / 基于激光显微切割的协议，用于神经黑色素颗粒蛋白质组学谱的LC-MS /…
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JoVE Journal Neuroscience
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基于激光显微切割的协议，用于神经黑色素颗粒蛋白质组学谱的LC-MS / MS分析

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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这里提出了一个强大的方案，用于通过激光显微切割从人死后黑质致密组织中分离神经黑色素颗粒。该修订和优化的方案大大减少了样品采集所需的时间，减少了所需的样品量，并通过LC-MS/MS分析增强了蛋白质的鉴定和定量。

Tags

神经科学，第178期，神经黑色素颗粒，激光显微切割，质谱， 黑质紧凑，平行反应监测，数据依赖性采集
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