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Journal / Neuroscience / Laser mikrodisseksjonsbasert protokoll for LC-MS…
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Neuroscience

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JoVE Journal Neuroscience
Laser mikrodisseksjonsbasert protokoll for LC-MS / MS-analyse av den proteomiske profilen til nevromelaningranulater
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Laser mikrodisseksjonsbasert protokoll for LC-MS / MS-analyse av den proteomiske profilen til nevromelaningranulater

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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En robust protokoll er presentert her for å isolere nevromelaningranuler fra humant post mortem substantia nigra pars compacta-vev via lasermikrodisseksjon. Denne reviderte og optimaliserte protokollen minimerer massivt den nødvendige tiden for prøveinnsamling, reduserer den nødvendige prøvemengden og forbedrer identifiseringen og kvantifiseringen av proteiner ved LC-MS / MS-analyse.

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Nevrovitenskap utgave 178 Neuromelaningranulat lasermikrodisseksjon massespektrometri substantia nigra pars compacta parallell reaksjonsovervåking dataavhengig innsamling
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