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Neuroscience

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JoVE Journal Neuroscience
Laser-Mikrodissektions-basiertes Protokoll für die LC-MS/MS-Analyse des proteomischen Profils von Neuromelanin-Granulaten
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Laser-Mikrodissektions-basiertes Protokoll für die LC-MS/MS-Analyse des proteomischen Profils von Neuromelanin-Granulaten

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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Hier wird ein robustes Protokoll zur Isolierung von Neuromelanin-Granulaten aus humanem postmortalem Substantia nigra pars compacta-Gewebe mittels Lasermikrodissektion vorgestellt. Dieses überarbeitete und optimierte Protokoll minimiert massiv die benötigte Zeit für die Probenentnahme, reduziert die erforderliche Probenmenge und verbessert die Identifizierung und Quantifizierung von Proteinen durch LC-MS/MS-Analyse.

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Neuroscience Ausgabe 178 Neuromelanin-Granula Lasermikrodissektion Massenspektrometrie Substantia nigra pars compacta paralleles Reaktionsmonitoring datenabhängige Erfassung
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