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JoVE Journal Neuroscience
Lasermikrodissektionsbaserat protokoll för LC-MS/MS-analys av den proteomiska profilen för neuromelaningranulat
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Lasermikrodissektionsbaserat protokoll för LC-MS/MS-analys av den proteomiska profilen för neuromelaningranulat

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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Ett robust protokoll presenteras här för att isolera neuromelaningranulat från human post-mortem substantia nigra pars compacta-vävnad via lasermikrodissektion. Detta reviderade och optimerade protokoll minimerar massivt den tid som krävs för provtagning, minskar den erforderliga provmängden och förbättrar identifieringen och kvantifieringen av proteiner genom LC-MS/MS-analys.

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Neurovetenskap utgåva 178 Neuromelaningranulat lasermikrodissektion masspektrometri substantia nigra pars compacta parallell reaktionsövervakning databeroende förvärv
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