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Journal / Neuroscience / Nöromelanin Granüllerinin Proteomik Profilinin…
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Neuroscience

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JoVE Journal Neuroscience
Nöromelanin Granüllerinin Proteomik Profilinin LC-MS/MS Analizi için Lazer Mikrodiseksiyon Tabanlı Protokol
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Nöromelanin Granüllerinin Proteomik Profilinin LC-MS/MS Analizi için Lazer Mikrodiseksiyon Tabanlı Protokol

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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Burada, nöromelanin granüllerinin lazer mikrodiseksiyonu yoluyla insan post-mortem substantia nigra pars compacta dokusundan izole edilmesi için sağlam bir protokol sunulmuştur. Bu revize edilmiş ve optimize edilmiş protokol, numune toplama için gereken süreyi büyük ölçüde en aza indirir, gerekli numune miktarını azaltır ve LC-MS / MS analizi ile proteinlerin tanımlanmasını ve nicelleştirilmesini geliştirir.

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Nörobilim Sayı 178 Nöromelanin granülleri lazer mikrodiseksiyonu kütle spektrometrisi substantia nigra pars compacta paralel reaksiyon izleme veriye bağımlı edinim
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