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Journal / Chemistry / 卡利内克特氏菌中神经肽的多方面质谱研究…
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Chemistry

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JoVE Journal Chemistry
卡利内克特氏菌中神经肽的多方面质谱研究
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卡利内克特氏菌中神经肽的多方面质谱研究

Article DOI: 10.3791/63322-v • 09:22 min • May 31st, 2022
May 31st, 2022 •
*1, *1, 1,2
1Department of Chemistry, University of Wisconsin-Madison, 2School of Pharmacy, University of Wisconsin-Madison
* These authors contributed equally

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Summary
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神经肽的质谱表征提供序列、定量和定位信息。这种优化的工作流程不仅对神经肽研究有用，而且对其他内源性肽也很有用。此处提供的方案描述了使用 LC-ESI-MS、MALDI-MS 点斑和 MALDI-MS 成像的神经肽的样品制备、MS 采集、MS 分析和神经肽的数据库生成。

Tags

化学，第183期，神经肽，质谱，肽纯化，同位素标记， 从头 测序，定量，LC，MALDI，质谱成像，数据库搜索，转录组挖掘
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