JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
从小鼠骨骼肌中分离线粒体用于呼吸测定
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠骨骼肌中分离线粒体用于呼吸测定
DOI:
10.3791/63336-v
•
08:11 min
•
February 10, 2022
•
Juan Diego Hernández-Camacho
2
,
Cristina Vicente-García
,
Ana Sánchez-Cuesta
2
,
Daniel J. M. Fernandez-Ayala
2
,
Jaime J. Carvajal
,
Plácido Navas
2
1
Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA
,
Universidad Pablo de Olavide
,
2
CIBERER
,
Instituto de Salud Carlos III
Chapters
00:05
Introduction
00:53
Muscle Dissection, Homogenization, and Mitochondrial Isolation
04:35
Preparation of the Microplate-Based Respirometric Assays
06:17
Results: Mitochondria Isolation from Mouse Skeletal Muscle
07:32
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们描述了从小鼠骨骼肌中分离线粒体的详细方法,以及随后使用基于微孔板的呼吸测定通过耗氧率(OCR)分析呼吸。该管道可用于研究多种环境或遗传干预措施对线粒体代谢的影响。
Tags
Mitochondria Isolation
Respirometric Assays
Skeletal Muscle
Mouse
Purification
Respiration Analysis
Experimental Conditions
Organism
Aging
Drug Testing
Cancer
Development
PBS
EDTA
IB1
Dissection
Skeletal Muscles
Tibialis Anterior
Extensor Digitorum Longus
Gastrocnemius
Soleus
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离
骨骼肌的线粒体分离
纯化的骨骼肌的先觉者
激光伤害的斑马鱼胚胎骨骼肌
单个肌纤维和成人骨骼肌卫星细胞的分离和培养
Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays
从人骨骼肌隔离血管源性多能前体
隔离和线粒体从培养细胞和小鼠组织功能分析
从小鼠骨骼肌高通量微孔板呼吸测量最低数量的隔离线粒体
骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动
Read Article