00:56Sample Intake, Heat Treatment, and Sample Assignment
01:52Operating Sample Loading Robots
04:09Manual Sample Loading
04:52Addition of Controls to Testing Plates
05:54Performing RT-qPCR and Determining Plate Validity
07:00Interpreting Sample Results
08:08Results: RT-qPCR Detection of N1 (SARS-CoV-2) Synthetic RNA and P1 (Hs_RPP30) Synthetic DNA, Comparison Between Manual and Automated Saliva Transfer N1 Ct Values, and Evaluation of Heat Treatment Methods for Viscosity Reduction in Saliva
Protokollen beskriver en SARS-CoV-2 diagnostisk metode, der bruger open source-automatisering til at udføre RT-qPCR molekylær test af spytprøver. Denne skalerbare tilgang kan anvendes til klinisk folkesundhedsovervågning samt til at øge kapaciteten i mindre universitetslaboratorier.