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从微解剖小鼠脉络丛中分离和表征免疫细胞
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JoVE Journal
Biology
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
Please note that all translations are automatically generated.
Click here for the English version.
从微解剖小鼠脉络丛中分离和表征免疫细胞
DOI:
10.3791/63487-v
•
09:55 min
•
February 03, 2022
•
Amaia Dominguez-Belloso
,
Sandrine Schmutz
,
Sophie Novault
,
Laetitia Travier*
1
,
Aleksandra Deczkowska*
1
1
Brain-Immune Communication Lab
,
Institut Pasteur
,
2
Cytometry platform, CB UTechS
,
Institut Pasteur
Chapters
00:05
Introduction
00:42
Dissection of the Choroid Plexus from the Brain
02:47
Preparation of Samples for Flow Cytometry Analysis
03:52
Choroid Plexus Myeloid Cells Gating
06:46
Choroid Plexus T Cells Gating
07:44
Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
09:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究使用流式细胞术和两种不同的门控策略对分离的灌注小鼠脑脉络丛;该协议确定了填充该大脑结构的主要免疫细胞亚群。
Tags
Immune Cells
Choroid Plexuses
Mouse Model
Brain Regulation
Cell Dissection
Cell Isolation
Cell Characterization
Collagenase IV
Lateral Ventricle
Third Ventricle
Fourth Ventricle
Cell Dissociation
MACS Buffer
Cell Centrifugation
Cell Washing
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