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Aislamiento y caracterización de las células inmunes de plexos coroideos de ratón microdisecados
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
DOI:

09:55 min

February 03, 2022

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Chapters

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Automatic Translation

Este estudio utiliza citometría de flujo y dos estrategias diferentes de gating en ratones perfundidos aislados plexos coroideos cerebrales; este protocolo identifica los principales subconjuntos de células inmunes que pueblan esta estructura cerebral.

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