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一种改进的袖带技术用于小鼠颈椎异位心脏移植模型
JoVE Journal
Medicine
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JoVE Journal
Medicine
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
Please note that all translations are automatically generated.
Click here for the English version.
一种改进的袖带技术用于小鼠颈椎异位心脏移植模型
DOI:
10.3791/63504-v
•
06:45 min
•
February 07, 2022
•
Xin Mao*
1
,
Peng Xian*
1
,
Hang You
2
,
Gang Huang
,
Jun Li
1
Department of Urological Oncology Surgery
,
Chongqing University Cancer Hospital
,
2
School of Medicine
,
Chongqing University
,
3
Department of Biochemistry and Molecular Biology, College of Basic Medical Science
,
Army Medical University
Chapters
00:05
Introduction
00:57
Recipient Procedure
03:04
Donor Procedure
04:44
Implantation
05:43
Results: Cervical Heterotopic Heart Transplantation in Mouse Model
06:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本协议中,使用小鼠心脏移植模型来研究心脏同种异体移植排斥的机制。在这种异位心脏移植模型中,提高了手术效率,并使用改进的袖带技术对心脏植入的颈椎端到端吻合确保了心脏移植物的存活。
Tags
Mouse Heart Transplantation
Cervical Heterotopic
Cuff Technique
Vessel Anastomosis
Cardiac Allograft
Immunologic Rejection
Surgical Procedure
Recipient Mouse
External Jugular Vein
Common Carotid Artery
Microvascular Clamp
Polyurethane Cuff
Suture
Heparin Saline
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