JoVE Journal
Biology
Biology
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セプチンの超微細構造組織、膜再形成、および曲率感度挙動をアッセイするためのボトムアップ インビトロ 法
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Article
DOI:
10.3791/63889-v
•
09:09 min
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August 17th, 2022
セプチンの超微細構造組織、膜再形成、および曲率感度挙動をアッセイするためのボトムアップ インビトロ 法
August 17th, 2022
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Brieuc Chauvin*1, Koyomi Nakazawa*1, Alexandre Beber1,7, Aurélie Di Cicco1, Bassam Hajj1, François Iv2, Manos Mavrakis2, Gijsje H. Koenderink3, João T. Cabral4, Michaël Trichet5, Stéphanie Mangenot*6, Aurélie Bertin*1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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セプチンは細胞骨格タンパク質である。それらは脂質膜と相互作用し、ミクロンスケールで膜曲率を感知するだけでなく生成することもできる。このプロトコルでは、膜変形、曲率に敏感なセプチン結合、およびセプチンフィラメントの超構造を分析するためのボトムアップ インビトロ 方法論について説明します。