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Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
DOI:

00:00 min

May 19, 2022

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

Automatic Translation

This protocol describes size exclusion chromatography, a facile and reproducible technique for enriching Mycobacterium tuberculosis extracellular vesicles from culture supernatants.

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