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結核 菌サイズ排除クロマトグラフィーによる細胞外小胞濃縮
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
DOI:

00:00 min

May 19, 2022

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

Automatic Translation

このプロトコールは、サイズ排除クロマトグラフィー、培養上清から 結核菌 群細胞外小胞を富化するための容易で再現性のある技術を記載する。

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