JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
結核
菌サイズ排除クロマトグラフィーによる細胞外小胞濃縮
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Mycobacterium tuberculosis
Extracellular Vesicle Enrichment through Size Exclusion Chromatography
Please note that all translations are automatically generated.
Click here for the English version.
結核
菌サイズ排除クロマトグラフィーによる細胞外小胞濃縮
DOI:
10.3791/63895-v
•
00:00 min
•
May 19, 2022
•
Joan M. Ryan
,
Karen M. Dobos
,
Nicole A. Kruh-Garcia
2
1
Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology
,
Colorado State University
,
2
BioMARC, Infectious Disease Research Center
,
Colorado State University
Chapters
00:04
Introduction
00:50
Preparation of Crude Mtb EV Concentrate
02:36
Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
05:52
Quantification and Qualification of the Mtb EVs
07:25
Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
08:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコールは、サイズ排除クロマトグラフィー、培養上清から
結核菌
群細胞外小胞を富化するための容易で再現性のある技術を記載する。
Tags
Mycobacterium Tuberculosis
Extracellular Vesicles
Size Exclusion Chromatography
Enrichment
Ultracentrifugation
Centrifugal Filter
PBS
BCA Assay
Size Exclusion Chromatography Column
AFC Tower
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
のサンプル調製<em>結核菌</em核磁気共鳴代謝研究のための>抽出
の成長
結核
バイオフィルム
自然送信時に結核マラリア重複感染を研究する実験モデル
結核菌
と
マラリアベルゲイ</i
多剤耐性を実証<em>結核菌</em>増幅マイクロアレイ
回復への新規マイクロダイセクションアプローチ<em>結核菌</em肺肉芽腫、ホルマリン固定パラフィン包埋から>特異的転写
上の機能的研究のための3Dヒト肺組織モデル<em>結核菌</em>感染
細胞内の標的阻害剤の有効性及び細胞毒性スクリーニングのためのシステム<em>結核菌</em
マクロファージ継代に対する薬効を評価するために、ハイスループットアッセイの互換性<em>結核菌</em
レポーター酵素蛍光を用いたマウスにおける
結核菌
をイメージング
マラリア感染時の細胞の小胞の機能評価
Read Article