Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Lentiviral medieret levering af shRNA'er til…
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Lentiviral medieret levering af shRNA'er til hESC'er og NPC'er ved hjælp af billig kationisk polymer polyethylenimin (PEI)
Microdissection of Black Widow Spider Silk-producing Glands
Journal (Biology)
Microdissection of Black Widow Spider Silk-producing Glands
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE
Journal (Biology)
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE
 
Click here for the English version

Lentiviral medieret levering af shRNA'er til hESC'er og NPC'er ved hjælp af billig kationisk polymer polyethylenimin (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Ved hjælp af den billige kationiske polymer polyethylenimin (PEI) producerede vi lentivirale partikler til stabil ekspression af shRNA'er i H9 humane embryonale stamceller (hESC'er) og forbigående transducerede H9-afledte neurale stamceller (NPC'er) ved høj effektivitet.

Tags

Biologi udgave 183 lentivirus shRNA hESC'er NPC'er polyethylenimin HEK293T-celler
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter