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Administración mediada por lentiviral de shRNAs a hESCs y NPCs usando polietileno de polímero catiónico de bajo costo (PEI)
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Administración mediada por lentiviral de shRNAs a hESCs y NPCs usando polietileno de polímero catiónico de bajo costo (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Utilizando el polímero catiónico de bajo costo polietilenimina (PEI), producimos partículas lentivirales para la expresión estable de shRNAs en células madre embrionarias humanas H9 (hESC) y células progenitoras neurales derivadas de H9 (NPC) transducidas transitoriamente con alta eficiencia.

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Biología Número 183 Lentivirus shRNA hESCs NPCs polietilenimina células HEK293T
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