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Lentivirral medierad leverans av shRNA till hESC och NPC med hjälp av billig katjonisk polymerpolyetylenimine (PEI)
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Lentivirral medierad leverans av shRNA till hESC och NPC med hjälp av billig katjonisk polymerpolyetylenimine (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Med hjälp av den billiga katjoniska polymeren polyetylenimin (PEI) producerade vi lentivirala partiklar för stabilt uttryck av shRNA i H9 humana embryonala stamceller (hESCs) och övergående transducerade H9-härledda neurala stamceller (NPC) med hög effektivitet.

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Biologi utgåva 183 Lentivirus shRNA hESCs NPC polyetylenimin HEK293T-celler
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