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JoVE Journal Biology
Consegna mediata lentivirale di shRNA a hESC e NPC utilizzando polietilenimina polimerica cationica a basso costo (PEI)
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Consegna mediata lentivirale di shRNA a hESC e NPC utilizzando polietilenimina polimerica cationica a basso costo (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Utilizzando il polimero cationico a basso costo polietilenimina (PEI), abbiamo prodotto particelle lentivirali per l'espressione stabile di shRNA in cellule staminali embrionali umane H9 (hESC) e cellule progenitrici neurali derivate da H9 trasdotte transitoriamente (NPC) ad alta efficienza.

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Biologia Numero 183 Lentivirus shRNA hESC NPC polietilenimina cellule HEK293T
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