Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Lentivirale gemedieerde levering van shRNA's aan…
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Lentivirale gemedieerde levering van shRNA's aan hESCs en NPC's met behulp van goedkope kationische polymeerpolyethylenimine (PEI)
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
Journal (Biology)
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
Journal (Biology)
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
 
Click here for the English version

Lentivirale gemedieerde levering van shRNA's aan hESCs en NPC's met behulp van goedkope kationische polymeerpolyethylenimine (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Met behulp van het goedkope kationische polymeer polyethylenimine (PEI) produceerden we lentivirale deeltjes voor stabiele expressie van shRNA's in H9 menselijke embryonale stamcellen (hESCs) en transiënt getransduceerde H9-afgeleide neurale voorlopercellen (NPC's) met een hoge efficiëntie.

Tags

Biologie Lentivirussen shRNA hESCs NPC's polyethylenimine HEK293T cellen
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter