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Journal / Biology / Lentiviral vermittelte Abgabe von shRNAs an hESCs …
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Lentiviral vermittelte Abgabe von shRNAs an hESCs und NPCs unter Verwendung des kostengünstigen kationischen Polymers Polyethylenimin (PEI)
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Lentiviral vermittelte Abgabe von shRNAs an hESCs und NPCs unter Verwendung des kostengünstigen kationischen Polymers Polyethylenimin (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Unter Verwendung des kostengünstigen kationischen Polymers Polyethylenimin (PEI) produzierten wir lentivirale Partikel für die stabile Expression von shRNAs in H9-humanen embryonalen Stammzellen (hES-Zellen) und transienten H9-abgeleiteten neuralen Vorläuferzellen (NPCs) mit hoher Effizienz.

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Biologie Ausgabe 183 Lentiviren shRNA hESCs NPCs Polyethylenimin HEK293T Zellen
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