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用于鉴定裂殖酵母多拷贝抑制因子的遗传筛选
JoVE Journal
Biology
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JoVE Journal Biology
Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
DOI:

13:22 min

September 13, 2022

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Chapters

  • 00:04Introduction
  • 01:00Transforming cDNA Library into the QuerySchizosaccharomyces pombe Mutant Strain and Screening Multicopy Suppressors
  • 05:16Validating the Suppressors of the Phenotype Associated with the Query Mutant Strain
  • 07:41Isolation of the Plasmid from the Schizosaccharomyces pombeSuppressor Clones
  • 09:55Identification of the Gene Encoded by the Suppressor Clone
  • 10:48Results: Screening Multicopy Suppressor of ell1 Deletion-Associated Genotoxic Stress Sensitivity
  • 12:49Conclusion

Summary

Automatic Translation

这项工作描述了 在Pombe裂殖酵母中进行多拷贝抑制遗传筛选的方案。该筛选使用全基因组质粒文库来鉴定与查询突变菌株相关的功能丧失表型的抑制克隆。使用该筛选鉴定 ell1 零突变体的新型遗传抑制因子。

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