JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
用于鉴定
裂殖酵母
多拷贝抑制因子的遗传筛选
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Genetic Screen for Identification of Multicopy Suppressors in
Schizosaccharomyces pombe
Please note that all translations are automatically generated.
Click here for the English version.
用于鉴定
裂殖酵母
多拷贝抑制因子的遗传筛选
DOI:
10.3791/63967-v
•
13:22 min
•
September 13, 2022
•
Vaibhav Bhardwaj*
1
,
Kumari Sweta*
1
,
Deepak Gyala
,
Nimisha Sharma
1
University School of Biotechnology, G.G.S. Indraprastha University
Chapters
00:04
Introduction
01:00
Transforming cDNA Library into the Query
Schizosaccharomyces pombe
Mutant Strain and Screening Multicopy Suppressors
05:16
Validating the Suppressors of the Phenotype Associated with the Query Mutant Strain
07:41
Isolation of the Plasmid from the
Schizosaccharomyces pombe
Suppressor Clones
09:55
Identification of the Gene Encoded by the Suppressor Clone
10:48
Results: Screening Multicopy Suppressor of ell1 Deletion-Associated Genotoxic Stress Sensitivity
12:49
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这项工作描述了
在Pombe裂殖酵母
中进行多拷贝抑制遗传筛选的方案。该筛选使用全基因组质粒文库来鉴定与查询突变菌株相关的功能丧失表型的抑制克隆。使用该筛选鉴定
ell1
零突变体的新型遗传抑制因子。
Tags
Genetic Screen
Multicopy Suppressors
Schizosaccharomyces Pombe
Genetic Interactions
Biological Pathways
Transformation Protocol
Ell1 Deletion Strain
Lithium Acetate TE
Carrier DNA
CDNA Library
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
光/暗转换测试小鼠
文化早期鸡胚体外(新文化)的方法
双整装原位杂交技术在早期鸡胚
整装在鸡的抗体染色方法
在鸡胚神经诱导的含量为
从成年小鼠海马齿状回的剖析
在毛发再生的上皮 - 间充质信号的快速遗传分析
成像生物膜损伤和参与亚细胞修复过程
化学遗传相互作用的快速鉴定<em>酿酒酵母</em
DNA电穿孔,肌纤维的分离和成像
Read Article