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在支持的脂质双层上重建膜系的最小肌动蛋白皮质
JoVE Journal
Biology
Author Produced
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JoVE Journal
Biology
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Please note that all translations are automatically generated.
Click here for the English version.
在支持的脂质双层上重建膜系的最小肌动蛋白皮质
DOI:
10.3791/63968-v
•
11:55 min
•
July 12, 2022
•
Darius Vasco Köster
,
Abrar Bhat
,
Sankarshan Talluri
,
Satyajit Mayor
1
Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School
,
University of Warwick
,
2
National Centre for Biological Sciences
,
Tata Institute of Fundamental Research
Chapters
00:05
Introduction
00:45
Preparation of Sample Chambers
02:59
Preparation of Supported Lipid Bilayers
04:31
Addition of Membrane-Actin Linker
05:27
Quality Assessment of the Lipid Bilayer
07:06
Polymerization and Addition of Fluorescent Actin Filaments
09:02
Addition of Myosin II
10:21
Results: Understanding the Membrane-Cortex Interface of Cells
11:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了支持的脂质双层的形成以及细胞骨架丝和运动蛋白的添加,以使用荧光显微镜研究重组,膜系留细胞骨架网络的动力学。
Tags
Membrane-tethered Actin Cortices
Supported Lipid Bilayers
Cytoskeletal Proteins
Reconstitution
Light Microscopy
Protein Assembly
Lipid Bilayer Formation
UV Curable Adhesive
Chamber Fabrication
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