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在支持的脂质双层上重建膜系的最小肌动蛋白皮质

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Please note that all translations are automatically generated. Click here for the English version.

在支持的脂质双层上重建膜系的最小肌动蛋白皮质

DOI:

10.3791/63968-v

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11:55 min

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July 12, 2022

•

Darius Vasco Köster, Abrar Bhat, Sankarshan Talluri, Satyajit Mayor

¹Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School,University of Warwick, ²National Centre for Biological Sciences,Tata Institute of Fundamental Research

Chapters

00:05Introduction
00:45Preparation of Sample Chambers
02:59Preparation of Supported Lipid Bilayers
04:31Addition of Membrane-Actin Linker
05:27Quality Assessment of the Lipid Bilayer
07:06Polymerization and Addition of Fluorescent Actin Filaments
09:02Addition of Myosin II
10:21Results: Understanding the Membrane-Cortex Interface of Cells
11:12Conclusion

Summary

Automatic Translation

该协议描述了支持的脂质双层的形成以及细胞骨架丝和运动蛋白的添加，以使用荧光显微镜研究重组，膜系留细胞骨架网络的动力学。

Tags

Keywords: Membrane-tethered Actin Cortices Supported Lipid Bilayers Cytoskeletal Proteins Reconstitution Light Microscopy Protein Assembly Lipid Bilayer Formation UV Curable Adhesive Chamber Fabrication

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