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Reconstitutie van membraangebonden minimale actinecorticosterces op ondersteunde lipidebilagen
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
DOI:

11:55 min

July 12, 2022

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Chapters

  • 00:05Introduction
  • 00:45Preparation of Sample Chambers
  • 02:59Preparation of Supported Lipid Bilayers
  • 04:31Addition of Membrane-Actin Linker
  • 05:27Quality Assessment of the Lipid Bilayer
  • 07:06Polymerization and Addition of Fluorescent Actin Filaments
  • 09:02Addition of Myosin II
  • 10:21Results: Understanding the Membrane-Cortex Interface of Cells
  • 11:12Conclusion

Summary

Automatic Translation

Dit protocol beschrijft de vorming van ondersteunde lipide bilayers en de toevoeging van cytoskeletale filamenten en motoreiwitten om de dynamiek van gereconstitueerde, membraangebonden cytoskeletale netwerken te bestuderen met behulp van fluorescentiemicroscopie.

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