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Biology

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Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
 

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Article doi: 10.3791/3187
November 18th, 2011

Summary November 18th, 2011

Microscopic imaging of live endothelial cells expressing GFP-actin allows characterization of dynamic changes in cytoskeletal structures. Unlike techniques that use fixed specimens, this method provides a detailed assessment of temporal changes in the actin cytoskeleton in the same cells before, during, and after various physical, pharmacological, or inflammatory stimuli.

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