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Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
DOI:

10:08 min

July 20, 2022

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Chapters

  • 00:04Introduction
  • 01:13Loading the Sample
  • 02:06Injecting the Oocytes
  • 02:49Recording of Ion Currents Using a Two-Electrode Voltage Clamp
  • 06:50Analyzing TEVC Recordings
  • 07:30Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
  • 09:25Conclusion

Summary

Automatic Translation

The protocol demonstrates that by performing microtransplantation of synaptic membranes into Xenopus laevis oocytes, it is possible to record consistent and reliable responses of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and γ-aminobutyric acid receptors.

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