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Approvvigionamento e perfusione-decellularizzazione di lembi vascolarizzati suini in un bioreattore di perfusione personalizzato
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1Latner Thoracic Surgery Research Laboratories,Toronto General Hospital Research Institute, University Health Network, 2Institute of Laboratory Medicine and Pathobiology,University of Toronto, 3Division of Thoracic Surgery, Department of Surgery,University of Toronto, 4Division of Plastic and Reconstructive Surgery, Department of Surgery,University of Toronto
Chapters
- 00:05Introduction
- 00:57Perfusion Bioreactor Fabrication
- 01:29Procurement of Porcine Flaps
- 05:51Setup of the Decellularization System
- 07:39Decellularization of Porcine Flaps and Evaluation of Decellularization
- 09:06Results: Perfusion-Decellularization of Porcine Vascularized Flaps
- 10:16Conclusion
Il protocollo descrive l'approvvigionamento chirurgico e la successiva decellularizzazione dei lembi suini vascolarizzati mediante la perfusione di detergente dodecilsolfato di sodio attraverso la vascolarizzazione del lembo in un bioreattore di perfusione personalizzato.
