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Magnetisk aktiveret celle sortering strategier for at isolere og rense Synovial væske-afledte mesenkymale stamceller fra en kanin Model
JoVE Journal
Biology
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JoVE Journal Biology
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
DOI:

10:20 min

August 10, 2018

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Chapters

  • 00:04Title
  • 01:07Isolate and Culture the rbSF-MSCs
  • 03:21CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture
  • 05:01Magnetic Separation and Culture of te CD90+ rbSF-MSCs
  • 06:44Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting
  • 08:39Conclusion

Summary

Automatic Translation

Denne artikel præsenterer en enkel og økonomiske protokol til ligetil isolering og oprensning af mesenkymale stamceller fra New Zealand hvid kanin synovial væske.

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