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Journal / Genetics / レンチウイルス媒介CRISPR/Cas9遺伝子編集を用いたノックアウト筋細胞株の開発…
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JoVE Journal Genetics
レンチウイルス媒介CRISPR/Cas9遺伝子編集を用いたノックアウト筋細胞株の開発
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Journal (Genetics)
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
Journal (Genetics)
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
 
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レンチウイルス媒介CRISPR/Cas9遺伝子編集を用いたノックアウト筋細胞株の開発

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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このプロトコルは、ガイドRNAの設計からノックアウトクローンの細胞クローニングおよび特性評価まで、CRISPR/Cas9を使用してノックアウト筋芽細胞を生成する方法を説明しています。

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遺伝学 第184号
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