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Journal / Genetics / Ontwikkeling van knock-out spiercellijnen met…
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Genetics

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JoVE Journal Genetics
Ontwikkeling van knock-out spiercellijnen met behulp van Lentivirus-gemedieerde CRISPR / Cas9-genbewerking
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Journal (Genetics)
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
Journal (Genetics)
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
 
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Ontwikkeling van knock-out spiercellijnen met behulp van Lentivirus-gemedieerde CRISPR / Cas9-genbewerking

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Het protocol beschrijft hoe knock-out myoblasten kunnen worden gegenereerd met CRISPR / Cas9, van het ontwerp van guide-RNA's tot het cellulair klonen en karakteriseren van de knock-out klonen.

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Genetica Nummer 184
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