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Desarrollo de líneas celulares muscularesknock-out utilizando la edición de genes CRISPR/Cas9 mediada por lentivirus…
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Genetics
Genetics
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Desarrollo de líneas celulares musculares knock-out utilizando la edición de genes CRISPR/Cas9 mediada por lentivirus
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Article
DOI:
10.3791/64114-v
•
10:12 min
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June 16th, 2022
Desarrollo de líneas celulares musculares knock-out utilizando la edición de genes CRISPR/Cas9 mediada por lentivirus
June 16th, 2022
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
El protocolo describe cómo generar mioblastos knock-out utilizando CRISPR/Cas9, desde el diseño de arneses guía hasta la clonación celular y caracterización de los clones knock-out.