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多路复用表面等离子体共振成像芯片上的多模态分析平台,用于分析细胞外囊泡亚群
JoVE Journal
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Biology
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Please note that all translations are automatically generated.
Click here for the English version.
多路复用表面等离子体共振成像芯片上的多模态分析平台,用于分析细胞外囊泡亚群
DOI:
10.3791/64210-v
•
06:12 min
•
March 17, 2023
•
Geetika Raizada
,
Balasubramaniam Namasivayam
,
Sameh Obeid
,
Benjamin Brunel
,
Wilfrid Boireau
,
Eric Lesniewska
,
Celine Elie-Caille
1
Université de Franche-Comté, CNRS UMR-6174
,
FEMTO-ST Institute
,
2
Lille Neuroscience & Cognition research centre
,
3
Institut Galien Paris-Saclay, CNRS UMR-8612 , Université Paris-Saclay
,
4
Interdisciplinary Lab Carnot Bourgogne LICB, CNRS UMR-6303
,
Université de Bourgogne Franche-Comté
Chapters
00:05
Introduction
00:48
Gold Substrate Preparation
01:35
Surface Plasmon Resonance Imaging
03:04
Atomic Force Microscopy
03:52
Results: Characterization of Extracellular Vesicles (EVs) on a Biochip
05:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了新一代多参数分析平台,具有更高的通量,用于表征细胞外囊泡亚群。该方法基于多重生物传感方法与原子力显微镜的计量和形态力学分析相结合,结合拉曼光谱,以鉴定捕获在微阵列生物芯片上的囊泡靶标。
Tags
Extracellular Vesicles
EVs
Multimodal Analytical Platform
Surface Plasmon Resonance Imaging
SPRi
Biochip
Label-free Detection
Nanomechanical Properties
Bioindicators
Drug Delivery
Biochip Preparation
Ligand Immobilization
Kinetics
Negative Control
Rat Serum Albumin
Ethanolamine
OG Wash
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