/
/
Cellulær affinitet av partikkelstabilisert emulsjon for å øke antigen internalisering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Graduate School of Bio-Applications and Systems Engineering,Tokyo University of Agriculture and Technology, 2Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 3State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,Chinese Academy of Sciences, 4University of Chinese Academy of Sciences
Chapters
- 00:05Introduction
- 01:01Preparation and Characterization of PLGA Nanoparticles
- 02:38Preparation and Characterization of PNPE
- 03:24bEVs Adhere to the PNPE
- 05:31CLSM Analysis of Antigen Uptake
- 08:14Results: Affinity of PNPE to bEVs and Uptake of Antigens
- 09:41Conclusion
For å rasjonelt designe effektive adjuvanser utviklet vi poly-melkesyre-koglykolsyre nanopartikkelstabilisert Pickering-emulsjon (PNPE). PNPE hadde unik mykhet og et hydrofobt grensesnitt for potent cellulær kontakt og tilbød antigenbelastning med høyt innhold, forbedret leveringssystemets cellulære affinitet til antigenpresenterende celler og induserte effektiv internalisering av antigener.
