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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Chapters
- 00:05Introduction
- 00:35Design and Construction of the sgRNA/crRNA Expression Cassette
- 01:47dCas12a and Ant‐CRISPR Protein Engineering and Plasmid Construction
- 02:38Immunofluorescence to Detect Cas Proteins
- 04:04Data Acquisition: FACS and Data Analysis
- 07:19Results: Efficiency of Both dCas9 and dCas12a‐Based Repressors and Activators in Yeast
- 10:20Conclusion
CRISPR-Cas systems and anti-CRISPR proteins were integrated into the scheme of Boolean gates in Saccharomyces cerevisiae. The new small logic circuits showed good performance and deepened the understanding of both dCas9/dCas12a-based transcription factors and the properties of anti-CRISPR proteins.
