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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Article DOI: 10.3791/64580-v • 07:48 min • October 11th, 2022
October 11th, 2022 •
*1, *1, 1,2, 1
1Department of Biology, Indiana University, 2Stowers Institute for Biomedical Research
* These authors contributed equally

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Summary
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Time-lapse microscopy is a valuable tool for studying meiosis in budding yeast. This protocol describes a method that combines cell-cycle synchronization, time-lapse microscopy, and conditional depletion of a target protein to demonstrate how to study the function of a specific protein during meiotic chromosome segregation.

Tags

Time-lapse Microscopy Stage-specific Nuclear Depletion Meiosis S. Cerevisiae Synchronized Cells Target Protein Myotic Mutant Budding Yeast Mitosis Nuclear Envelope Breakdown Cell Cycle Events Agar Pad Imaging Microcentrifuge Tube Agar Solution Yeast Cells Sporulation Culture
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