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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

DOI:
10.3791/64580-v

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07:48 min

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October 11, 2022

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, , ,
1Department of Biology,Indiana University, 2Stowers Institute for Biomedical Research

Chapters

  • 00:05Introduction
  • 00:42Time-Lapse Fluorescence Microscopy
  • 05:32Analysis of Chromatin Segregation
  • 06:18Results: Ctf19 Anchor Away Time and Chromatin Segregation
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Time-lapse microscopy is a valuable tool for studying meiosis in budding yeast. This protocol describes a method that combines cell-cycle synchronization, time-lapse microscopy, and conditional depletion of a target protein to demonstrate how to study the function of a specific protein during meiotic chromosome segregation.

Tags

Keywords: Time-lapse MicroscopyStage-specific Nuclear DepletionMeiosisSaccharomyces CerevisiaeProphase IConditional Protein DepletionBudding Yeast MitosisCell Cycle EventsNuclear Envelope BreakdownAgar PadMonolayer Of CellsSporulation CultureCentrifugation

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