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Biology
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zSpRY-ABE8eを用いたゼブラフィッシュヒト遺伝病モデリングのための効率的なPAMレスベース編集
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JoVE Journal
Biology
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
Please note that all translations are automatically generated.
Click here for the English version.
zSpRY-ABE8eを用いたゼブラフィッシュヒト遺伝病モデリングのための効率的なPAMレスベース編集
DOI:
10.3791/64977-v
•
07:31 min
•
February 17, 2023
•
Shaohui Zheng
2
,
Fang Liang
,
Yu Zhang
2
,
Ji-Feng Fei
,
Wei Qin
2
,
Yanmei Liu
2
1
Key Laboratory of Brain, Cognition and Education Science
,
Ministry of Education
,
2
Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science
,
South China Normal University
,
3
Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences
,
South China Normal University
,
4
Department of Pathology, Guangdong Provincial People’s Hospital
,
Guangdong Academy of Medical Sciences
Chapters
00:04
Introduction
01:02
Preparation of Microinjection Capillaries and Modified RNA
02:23
RNA Microinjection of Zebrafish Embryos
04:36
Efficiency Analysis of EditR Base Edits
06:08
Results: TSR2 Diamond Blackfan Zebrafish Mutation
06:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、zSpRY-ABE8eを使用して正確なゼブラフィッシュ病モデルを構築するために、PAMの制限なしに効率的なアデニン塩基編集を実行する方法について説明します。
Tags
Keywords: PAM-less Base Editing
ZSpRY-ABE8e
Zebrafish Modeling
Single-nucleotide Variants
Disease Mechanisms
Drug Screening
Micropipette Puller
MRNA
SgRNA
Pneumatic Microinjector
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