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全细胞记录的光在视网膜切片诱发兴奋性突触电流
JoVE Journal
Biology
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JoVE Journal Biology
Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
DOI:

19:33 min

July 02, 2008

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Chapters

  • 00:00Title
  • 00:10Preparations for the Dissection
  • 02:40Dissection of the Eye
  • 09:42Moving Slices to the Recording Chamber
  • 12:08Preparation of patch Electrodes
  • 13:21The Recording Rig
  • 14:09Whole-cell Recording
  • 17:40Recording Light-evoked Currents
  • 18:55Discussion/ Conclusion

Summary

Automatic Translation

录像显示,水产品虎蝾螈视网膜片全细胞电压钳记录的过程。我们示范片的准备,以及如何执行膜片钳记录在视网膜的视觉刺激。

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