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Biology
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双整装原位杂交技术在早期鸡胚
JoVE Journal
Biology
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JoVE Journal
Biology
Double Whole Mount in situ Hybridization of Early Chick Embryos
Please note that all translations are automatically generated.
Click here for the English version.
双整装原位杂交技术在早期鸡胚
DOI:
10.3791/904-v
•
15:42 min
•
October 27, 2008
•
Delphine Psychoyos
,
Richard Finnell
1
Center for Environmental and Genetic Medicine
,
Institute of Biosciences and Technology – Texas A&M Health Science Center
,
2
Center for Environmental and Genetic Medicine
,
Texas A&M University (TAMU)
Chapters
00:00
Title
00:49
Introduction
01:16
Fixing and Dehydrating the Embryos
03:28
Prehybridzation
05:15
Hybridizing Embryos with DIG- and FITC- Coupled Probes
06:41
DIG Antibody Incubation and Color Reaction
09:40
FITC Antibody Incubation and Color Reaction
11:52
Fixation and Processing
13:34
Results
15:08
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
本视频演示了原位杂交的方法,其中2个不同的基因的时空表达模式可以在年轻的鸡胚显示2色整装。这种方法最初是由大卫威尔金森,多明戈斯恩里克,菲尔英厄姆和大卫ISH - Horowicz。
Tags
Double Whole Mount In Situ Hybridization
Early Chick Embryos
Embryonic Development
Gene Expression
Brain Development
Neural Tube Formation
Somite Formation
Heart Primordia Formation
2-color Whole Mount In Situ Hybridization
Embryo Dissection
Paraformaldehyde Fixation
Prehybridization
Probe Hybridization
DIG Probe
FITC Probe
Overnight Hybridization
DIG Coupled Antibody
FITC Coupled Antibody
Color Reaction For DIG Substrate
Color Reaction With FITC
Photograph And Sectioning
Troubleshooting Guide
Article
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