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Chapters
- 00:00Title
- 00:49Introduction
- 01:16Fixing and Dehydrating the Embryos
- 03:28Prehybridzation
- 05:15Hybridizing Embryos with DIG- and FITC- Coupled Probes
- 06:41DIG Antibody Incubation and Color Reaction
- 09:40FITC Antibody Incubation and Color Reaction
- 11:52Fixation and Processing
- 13:34Results
- 15:08Conclusion
Questo video dimostra a 2 colori montare tutto ibridazione in situ, un metodo con cui il modello di espressione spaziale e temporale dei 2 geni differenti possono essere visualizzati in embrioni di pollo giovani. Questo metodo è stato originariamente introdotto da David Wilkinson, Domingos Henrique, Phil Ingham e David Ish-Horowicz.
Tags
Double Whole Mount In Situ HybridizationEarly Chick EmbryosEmbryonic DevelopmentGene ExpressionBrain DevelopmentNeural Tube FormationSomite FormationHeart Primordia Formation2-color Whole Mount In Situ HybridizationEmbryo DissectionParaformaldehyde FixationPrehybridizationProbe HybridizationDIG ProbeFITC ProbeOvernight HybridizationDIG Coupled AntibodyFITC Coupled AntibodyColor Reaction For DIG SubstrateColor Reaction With FITCPhotograph And SectioningTroubleshooting Guide
