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补丁钳爪蟾卵母细胞中表达的离子通道
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Biology
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JoVE Journal
Biology
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
Please note that all translations are automatically generated.
Click here for the English version.
补丁钳爪蟾卵母细胞中表达的离子通道
DOI:
10.3791/936-v
•
08:00 min
•
October 16, 2008
•
Austin L Brown
,
Brandon E. Johnson
,
Miriam B. Goodman
1
Department of Molecular and Cellular Physiology
,
Stanford University
,
2
Department of Molecular and Cellular Physiology
,
Stanford University School of Medicine
Chapters
00:00
Title
00:49
Introduction
01:35
Removing the Vitelline Membrane
03:33
Gigaseal Formation
06:05
Outside-Out Topology
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这是为了介绍，从非洲爪蟾卵母细胞来修补钳记录。它涵盖了卵黄膜去除，形成一个gigaohm密封（gigaseal），和可选的补丁外拓扑的转换。
Tags
Patch Clamp Recording
Ion Channels
Xenopus Oocytes
Electrophysiological Measurement
Heterologous Cells
African Clawed Frog
Xenopus Laevis
Macropatch
Single-channel Recording
Oocyte Preparation
Seal Generation
Successful Seal Generation
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