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改进的膜片钳记录的压力抛光移液器
JoVE Journal
Biology
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JoVE Journal
Biology
Pressure-polishing Pipettes for Improved Patch-clamp Recording
Please note that all translations are automatically generated.
Click here for the English version.
改进的膜片钳记录的压力抛光移液器
DOI:
10.3791/964-v
•
05:12 min
•
October 22, 2008
•
Brandon E. Johnson
,
Austin L. Brown
,
Miriam B. Goodman
1
Department of Molecular and Cellular Physiology
,
Stanford University School of Medicine
Chapters
00:00
Title
00:31
Introduction
01:00
Pulling and Pressure Polishing Pipettes
04:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这是一个指导修改形状的玻璃微量。具体来说,通过热和空气压力的锥度不增加尖端张开扩大,导致较低的吸液管电阻。这是至关重要的,以获得小细胞低噪音录音,但在许多应用中非常有用。
Tags
Pressure-polishing
Pipettes
Patch-clamp Recording
Glass Pipettes
Shaping
Fabricating
Neuronal Cell Bodies
Glass-blowing
Air Pressure
Heat
Shape Modification
Micropipette Puller
Soft Glass
Hard Glass
Pipette Resistance
Tip Opening Diameter
Soda Lime Glass
Borosilicate Glass
High-pressure Valve
Microforge
Ultrasmall Cells
Single-channel Recording
Blunt Shape
Perforated-patch Clamp Recording
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