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产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养
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Biology
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JoVE Journal
Biology
Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
Please note that all translations are automatically generated.
Click here for the English version.
产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养
DOI:
10.3791/990-v
•
16:04 min
•
January 16, 2009
•
Hae Young Lee
,
Lloyd A. Greene
,
Carol A. Mason
3
,
M. Chiara Manzini
4
1
Department of Genetics and Development
,
Columbia University
,
2
Department of Pathology and Cell Biology
,
Columbia University
,
3
Department of Neuroscience
,
Columbia University
,
4
Department of Neurology, Beth Israel Deaconess Medical Center
,
Harvard Medical School
Chapters
00:00
Title
01:10
Introduction
01:37
Preparing for Cerebellar Dissection
03:15
Cerebellar Dissection and Meninges Removal
06:01
Cerebellar Dissection and Meninges Removal
09:29
Percoll Gradient Separation
12:03
Pre-plating and Plating
13:23
Results
14:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个方法来隔离和文化的小脑颗粒神经元祖细胞和产后小鼠小脑颗粒神经元。
Tags
Isolation
Culture
Post-natal
Mouse Cerebellar
Granule Neuron Progenitor Cells
Neurons
Cerebellar Cortex
Neuronal Properties
Development
Granule Neurons
Purkinje Cells
Inhibitory Interneurons
Mammalian Brain
Rodents
External Granule Layer (EGL)
Enrichment
Purity
Differentiation
Proliferating Progenitor Cells
Synaptogenesis
Glutamate Receptor Function
Interaction With Other Purified Cerebellar Cells
Cell Death
Article
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