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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
JoVE Journal
Biology
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JoVE Journal Biology
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
DOI:

19:16 min

August 05, 2009

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Chapters

  • 00:05Title
  • 00:58Introduction
  • 01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
  • 05:46Microinjection of Labeled Actin
  • 10:14Assembling the Imaging Chamber
  • 15:20Imaging Cells
  • 18:17Representative Results: Microinjected Cells
  • 18:44Conclusion

Summary

Automatic Translation

Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

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