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In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
JoVE Journal
Biology
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JoVE Journal Biology
In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
DOI:

10:27 min

August 18, 2009

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Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:25Bee Preparation and Neuron Staining
  • 04:28In vivo Imaging
  • 06:50Odor Stimulation and Recording of Signals
  • 08:00Morphological Anaylsis and Reconstruction
  • 09:24Representative Calcium Imaging and Confocal Scanning Results
  • 10:22Conclusion

Summary

Automatic Translation

Bees can be conditioned in an appetitive olfactory learning paradigm (PER-conditioning). Using odors as stimuli, we established a method in which behavior is recorded while simultaneously Calcium Imaging is used to measure odor evoked activity in mushroom body neurons in vivo.

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