高純度の取得トキソプラズマオーシスト

Immunology and Infection
 

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Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

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Abstract

References

  1. Dubey, J. P. Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers. J. Parasitol. 91, 1082-1093 (2005).
  2. Tenter, A. M., Heckeroth, A. R., Weiss, L. M. Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217-1258 (2000).
  3. Bowie, W. R. Outbreak of toxoplasmosis associated with municipal drinking water. Lancet. 350, 173-177 (1997).
  4. Moura, L. de Waterborne toxoplasmosis, Brazil, from field to gene. Emerg. Infect. Dis. 12, 326-329 (2006).
  5. Jones, J. L., Dubey, J. P. Waterborne toxoplasmosis - Recent developments. Exp. Parasitol. doi: 10.1016/j.exppara.2009.03.013, (2009).
  6. Dumetre, A., Darde, M. L. How to detect Toxoplasma gondii oocysts in environmental samples. FEMS Microbiol. Rev. 27, 651-661 (2003).
  7. Dumetre, A., Darde, M. L. Purification of Toxoplasma gondii oocysts by cesium chloride gradient. J. Microbiol. Methods. 56, 427-4230 (2004).
  8. Dubey, J. P. Toxoplasmosis of Animals and Man. 2nd Edition, CRC Press, Inc. Boca Raton, FL. (2009).

Comments

2 Comments

  1. Good protocol for study of Toxoplasma gondii!

    Reply
    Posted by: Anonymous
    April 17, 2011 - 10:07 AM
  2. Dear Dr.Sarah,I want to use this protocol to purify the toxoplasma cyst.Does this protocol useful to purify the Toxoplasma cyst?

    Reply
    Posted by: weisheng c.
    July 4, 2014 - 1:09 AM

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