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Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
JoVE Journal
Biology
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JoVE Journal Biology
Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
DOI:

15:59 min

April 30, 2010

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Chapters

  • 00:00Title
  • 02:55Genetic Cross
  • 04:55Treatment of Frames
  • 06:12Laser Microdissector Set-Up
  • 07:42Collection of Wing Discs from Drosophila Third instar Transgenic Larvae
  • 09:43Laser MicroDissection
  • 13:21Transcription Profiling
  • 15:27Credits

Summary

Automatic Translation

Laser microdissection was applied to analyse gene expression profiling in specific compartments of Drosophila wing disc subjected to localised RNAi in vivo. RNA extracted from equivalent areas of silenced and unsilenced compartments was analysed by quantitative RT-PCR to determine comparative gene expression profiling within the context of native tissue microecology.

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