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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

DOI:
10.3791/1963-v

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10:39 min

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June 12, 2010

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,
1Dept. Of Cell Biology and Molecular Genetics,University of Maryland

Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:24Preparing for Bombarding the Cells
  • 03:32Bombardment
  • 07:16Observation
  • 08:50Representative Images of the BiFC Assay in Onion Cells Transfected with the Helios Gene Gun
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Tags

Bimolecular Fluorescence ComplementationBiFC AssayProtein-protein InteractionOnion CellsHelios Gene GunGene FunctionFluorescent ProteinGFPPlant ResearchersOnion Epidermal CellsPlasmid DNAArabidopsis ThalianaSEUSSLEUNIG HOMOLOG

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