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Quantitation of γH2AX Foci in Tissue Samples
JoVE Journal
Biology
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JoVE Journal Biology
Quantitation of γH2AX Foci in Tissue Samples
DOI:

08:48 min

June 28, 2010

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Chapters

  • 00:00Title
  • 00:05Introduction
  • 01:42Tissue Preparation
  • 02:29Immunofluorescence Staining
  • 05:19Microscopy
  • 07:02Analysis
  • 08:24Conclusion

Summary

Automatic Translation

Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.

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