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Journal
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Biology
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RhoC GTPase Activation Assay
JoVE Journal
Biology
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JoVE Journal
Biology
RhoC GTPase Activation Assay
RhoC GTPase Activation Assay
DOI:
10.3791/2083-v
•
09:58 min
•
August 22, 2010
•
Michelle Lucey
,
Heather Unger
,
Kenneth L. van Golen
1
Department of Biological Sciences, The Center for Translational Cancer Research
,
University of Delaware
Chapters
00:00
Title
00:48
Introduction
01:25
Preparation of GST-fusion Protein
04:41
GST-RBD Pull Down Assay
09:01
Representative Results for a RhoC GTPase Activation Assay
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
This protocol utilizes a pull down assay to determine the levels of active RhoC GTPase within cells.
Tags
RhoC GTPase
RhoA GTPase
Activation Assay
Homology
Reagents
Techniques
Metastatic Cancer Cells
Rho Activation Assay
GST-Rho Binding Domain Fusion Protein
Active RhoC GTPase
Total Protein
GTP Bound
GDP Bound
Commercial Versions
Commercial Kits
RhoC-specific Antibody
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